Results
| PMID | 23287096 |
| Gene Name | OGG1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 44 |
| Population details | 44 (14 stage I/II endometriosis, 16 stage III/IV endometriosis, 14 controls) |
| Sex | Female |
| Other associated phenotypes |
Endometriosis |
|
Reprod Sci. 2013 Jun;20(6):688-98. doi: 10.1177/1933719112466301. Epub 2013 Jan Carvalho, Luiz Fernando Pina| Abrao, Mauricio Simoes| Biscotti, Charles| Sharma, Rakesh| Nutter, Benjamin| Falcone, Tommaso 1Center for Reproductive Medicine, Obstetrics and Gynecology and Women's Health Institute, Cleveland Clinic, Cleveland, OH 44195, USA. BACKGROUND: There is increasing evidence that oxidative stress is one of the key factors for progression of endometriosis. In this prospective controlled trial, we measured 6 different biomarkers of oxidative stress targeting protein, lipid, and DNA to quantify the severity and progression of endometriosis and establish a diagnostic marker for the disease. METHODS: A total of 62 consecutive patients were identified and enrolled in this study. After exclusion criteria, 44 patients were allocated to 3 groups: stage I/II (n = 14), stage III/IV (n = 16), and a control group (n = 14). The levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-oxoguanine DNA glycosylase (OGG1), protein carbonyl (PC), lipid peroxidation (LPO), reactive oxygen species (ROS), and total antioxidant capacity (TAC) were accessed in peritoneal fluid and tissue. RESULTS: Significantly higher levels of 8-OHdG and PC were seen in patients with endometriosis, in addition OGG1 expression was found to be significantly lower in patients with endometriosis (P < .001, P = .001, P = .033, respectively); ROS, TAC, and LPO were similar in stages I/II, stages III/IV, and control group. A predictive model was built using multivariable analyses and receiver-operating characteristics curves. The ability to predict and distinguish between patients without endometriosis, stage I/II endometriosis, and stage III/IV was very high. This model was highly discriminatory and had a concordance index of 0.87. CONCLUSION: In this cohort, higher DNA damage and lower DNA repair activity was related to endometriosis progression. Our results indicate that oxidative stress as a biomarker of cell injury can be used as a reliable quantitative test of endometriosis severity. Mesh Terms: Adult| Ascitic Fluid/chemistry| Biomarkers/analysis| Biopsy| Case-Control Studies| DNA Damage| DNA Glycosylases/analysis| DNA Repair| Deoxyguanosine/analogs & derivatives/analysis| Disease Progression| Endometriosis/genetics/*metabolism/patholog |
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